Application

Research Sub CategoryCytoskeleton

Anti-Cortactin Antibody, phospho-specific (Tyr421) detects level of Cortactin & has been published & validated for use in WB.

Western blot: 1-2 µg/mL

Optimal working dilutions must be determined by end user.

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Rabbit anti-Cortactin (Y421) polyclonal antibody was raised against a peptide corresponding to residues surrounding phosphorylated Y421of human cortactin.

Epitope: phospho-specific (Tyr421)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Liquid in 1mg/mL in PBS with 0.05% Bronidox.

Specificity

Recognizes Cortactin phosphorylated on tyrosine 421. Cortactin is an 80-85 kDa actin-binding protein, which consists of a variety of protein domains. The nature and function of these domains, coupled with the presence of multiple consensus sites for phosphorylation by tyrosine and serine/threonine kinases, suggests that cortactin integrates activation of specific signaling pathways with cytoskeletal reorganization.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months after receipt.